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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in many tumors, which results in chromosome instability, and thus could lead to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a critical role in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been explained. Two more splice variants have been noted but detailed nucleotide sequence information will not be but accessible.
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Blocking peptides are peptides that bind specifically towards the focus on antibody and block antibody binding. These peptide ordinarily consists of the epitope acknowledged with the antibody.
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This tutorial outlines further more possible optimization of cellular and immunoassay parameters to make sure the best possible final results are...
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This guideline outlines further feasible optimization of cellular and immunoassay parameters to ensure the absolute best final results are...
Antibodies sure to the blocking peptide no longer bind for the epitope within the goal protein. By comparing the staining through the blocked antibody compared to the antibody by yourself, you can see which staining is certain.
Focus on Info Cyclin E belongs to the really conserved cyclin family, whose associates are characterised by a remarkable periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation patterns which add for the temporal coordination of each and every mitotic event. Cyclin E kinds a fancy with and features as being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both check here antibodies...
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